Apostle MiniMaxTM Technology


Overview

Apostle MiniMaxTM technology offers a revolutionary 10%-10X higher efficiency to capture and isolate the circulating cell-free genetic materials.

The ability to isolate and analyze circulating cell free DNA (cfDNA) at very low concentrations is becoming increasingly important, particularly in non-invasive prenatal test (NIPT), early cancer detection, and infectious disease diagnosis. Highly efficient isolation of cfDNA from complexed biological medium is a crucial step for subsequent cfDNA analysis.

Products
Products Cat# User manual List price (USD) Order
Apostle MiniMaxTM High Efficiency Cell-Free DNA Isolation Kit (Standard Edition) (25-50 preps) A17622-50 Protocol (v27) $498.00 Order
Apostle MiniMaxTM High Efficiency Cell-Free DNA Isolation Kit (Standard Edition) (5-10 preps) A17622-10 Protocol (v27) $125.00 Order
Apostle MiniMaxTM High Efficiency Magnetic Nanoparticles (Standard Edition) (5mL) A320 $800.00 Order
Apostle MiniMaxTM High Efficiency Cell-Free DNA Isolation Kit (Type S) (25-50 preps) A17830-50 Protocol (v20) $598.00 Order
Apostle MiniMaxTM High Efficiency Cell-Free DNA Isolation Kit (Type S) (5-10 preps) A17830-10 Protocol (v20) $150.00 Order
Apostle MiniMaxTM High Efficiency Magnetic Nanoparticles (Type S) (5mL) A321 $950.00 Order
Why important?

A recent paper published in JAMA Oncology by Torga & Pienta revealed that, strikingly, 2 major commercial liquid biopsy tests show significant and clinically unacceptable discordance.

Why do liquid biopsy tests differ so significantly? Are they reliable?

Among other impact factors, the extraction method of circulating free DNA (cfDNA) plays a major role here. Data show that different cfDNA extraction methods yield significantly different amounts of cfDNA by 10% to 10 folds, and consequently result in discordant conclusions. When we study genomic DNA isolated from the nucleus of cells, this level of difference may not matter much – because the amount of genetic material commonly reaches hundreds of micrograms (ug), so the downstream testing methods have enough genetic material to work with anyway. However, when we study cfDNA, this level of difference may result in a fundamental discordance, because the amount of cfDNA is commonly less than 100 nanograms (ng). Slight difference of cfDNA yields may result in critical impact on the testable copies of cancer mutations and the signal-to-noise ratio.

We illustrate how the accuracy of liquid biopsy is impacted by cfDNA extraction in a figure below. To have a reliable liquid biopsy testing, a highly efficient cfDNA extraction must be achieved.

MiniMax data image

Exhibit 1. How the accuracy of liquid biopsy is impacted by cfDNA extraction.

What unique?

Apostle MiniMax technology ensures precise capture and separation of circulating genetic materials for liquid biopsy analysis. This is achieved through Apostle’s novel proprietary MiniMax magnetic nanoparticles (Exhibit 2-8) with innovative features:

  • Novel material composition and surface chemistry - completely distinct from the conventional paramagnetic or superparamagnetic technologies
  • Exceptionally large surface area
  • Minimized variation
  • Best-in-class suspension property
  • Superb magnetic power
  • Superb resistance to particle clustering

MiniMax data image

Exhibit 2. Apostle’s proprietary MiniMax magnetic nanoparticles under scanning electron microscope.

The Apostle MiniMax nanoparticles have an increased magnetic strength and a decreased particle size compared to other leading technologies in the market, which ensures excellent suspension in solution and rapid mobility. The optimized surface chemistry allows efficient enrichment of genetic materials from complex biological materials.
MiniMax data image

Exhibit 3. Apostle’s proprietary MiniMax nanoparticles have uniform sizes.

Apostle’s MiniMax magnetic nanoparticles generated from our proprietary technology have a uniform size distribution with minimized doublets, distinct from the particles from five current technological providers showing random sizes and significant doublets. Highly consistent size distribution of Apostle’s nanoparticles ensure reproducible results.

Exhibit 4. (YouTube Video). The Apostle MiniMax nanoparticles have a best-in-class suspension property.

Excellent suspension is one of the critical properties of nanoparticles to excel in cfDNA isolation. In this simple but quite visual experiment, we compare Apostle MiniMax with the nanoparticles from other two technologies on the market. The three tubes contain equal weight of different types of nanoparticles.
MiniMax data image

Exhibit 5 (Photo). The Apostle MiniMax nanoparticles have a best-in-class suspension property.

The three tubes contain equal weight of different types of nanoparticles. By 1 minute, the other technology #2 is almost completely sedimented. By 15 minute, the other technology #1 is almost completely sedimented. However, Apostle MiniMax has kept the suspension status, showing a superb suspension property.

Exhibit 6 (YouTube Video). The Apostle MiniMax nanoparticles have a best-in-class magnetic power.

A strong magnetic power is another critical property of nanoparticles to achieve a good cfDNA isolation performance. In this simple experiment, we compare the magnatic power of Apostle MiniMax with the nanoparticles from other two technologies on the market. The three tubes contain equal weight of different types of nanoparticles. A magnetic plate slowly approaches the three tubes at equal distances. Whichever tube having the strongest magnetic power makes the first move.
The tube containing Apostle MiniMax makes the first move, while the other two technologies stay still. Apostle MiniMax shows a superb magnetic power.
MiniMax data image

Exhibit 7 (Photo). The Apostle MiniMax nanoparticles have a best-in-class magnetic power.

The three tubes contain equal weight of different types of nanoparticles. A magnetic plate slowly approaches the three tubes at equal distances. Whichever tube having the strongest magnetic power makes the first move.
The tube containing Apostle MiniMax makes the first move, while the other two technologies stay still. Apostle MiniMax shows a superb magnetic power.

MiniMax data image

Exhibit 8 (Photo). The Apostle MiniMax nanoparticles have a superb resistance to particle clustering.

The unwanted clustering of particles reduces the performance of the cfDNA isolation, and sometimes even interferes with the normal lab procedures. In this experiment, we compare MiniMax's ability to resist clustering with another leading technology on the market. After adding isopropyl alcohol, the other technology shows particle clustering visible to naked eye, while Apostle MiniMax does not. The Apostle MiniMax nanoparticles show a superb resistance to particle clustering.

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