Apostle MiniEnrichTM Carboxyl Beads for Size Selection and Purification

Next-generation purification, size selection, and long-read size selection
For research use only. Not for use in diagnostic procedures.


Product Cat# User manual
Apostle MiniEnrichTM Size Selection Beads A190606 Protocol
Apostle MiniEnrichTM Purification Beads A190607 Protocol


Apostle MiniEnrich data image Apostle MiniEnrich data image

  • Novel magnetic nanoparticles structure and proprietary surface chemistry
  • Compatible with current workflows
  • Enhanced performance for specific removal of non-target fragments
  • Significantly improved magnetic response for less pelleting time and less beads carry-over


Apostle MiniEnrich data image

Apostle MiniEnrichTM Carboxyl Beads for size selection and purification offers strong magnetic response.

Apostle MiniEnrichTM Carboxyl Beads are pelleted against the magnet within 25 seconds, while the market leading product takes 2 minutes and 40 seconds.



Apostle MiniEnrichTM Size Selection Beads

Designed for precise size selection for NGS


Introduction

The Apostle MiniEnrichTM Size Selection Beads is designed for size selection of DNA fragments from a mixture of DNA with various sizes, which potentially benefits downstream assays like NGS and other molecular biology workflows. It can be used on samples before or after DNA fragmentation, ligation, or library amplification during NGS library preparation workflow. With proprietary Apostle MiniEnrichTM technology, the kit is featured for customized cut-off size, efficient removal of non-target fragments and recovery of target DNA fragments.

Performance Evaluation

Apostle MiniEnrichTM Size Selection Beads is designed to precisely select DNA based on its size. It offers less DNA residue of non-target fragments, and selects DNA fragments in narrower size range, resulting sharp peak with good resolution and high yield for NGS workflows (Exhibit 1, 2, and 3).

Apostle MiniEnrich data image

Exhibit 1. Less DNA residue results sharp peak with good resolution and high yield.

dsDNA step ladder (50-3000 bp) in a TE buffer (red) was treated with Apostle MiniEnrichTM Size Selection Beads for left-side size selection (blue) or a magnetic beads-based market-leading product (green). Comparing to market-leading product, Apostle MiniEnrichTM Size Selection Beads offers less DNA residue of non-target fragments (>500 bp), while preserves fragments of interest (<500 bp) with high yield. Less DNA residue of non-target fragments results narrower size selection range, and sharp peak with good resolution and high yield.

Exhibit 2. Adjust beads to sample ratio for customized size selection.

gDNA from Hela cells was fragmented by NEBNext® dsDNA Fragmentase® (red) and Apostle MiniEnrich Size Selection Beads was used to select DNA fragments of interest. By adjusting the beads to sample ratio, the cut-off can be achieved with good yield and resolution.
Apostle MiniEnrich data image

Apostle MiniEnrich data image

Exhibit 3. Size Selection for long fragments for long-read NGS and other applications.

dsDNA step ladder (50-3000 bp) in a TE buffer (blue) was treated with Apostle MiniEnrichTM Size Selection Beads for right-side size selection (red). Comparing with DNA input (blue), Apostle MiniEnrichTM Size Selection Beads selects long DNA fragments (>1000 bp) from short fragments with good yield and resolution, which expands size selection to long-read sequencing, gaining more insights of samples.



Apostle MiniEnrichTM Purification Beads

Designed for better, easier and specific removal of non-target fragments for NGS and PCR workflows


Introduction

The Apostle MiniEnrichTM Purification Beads is designed for purification and clean-up of DNA fragments from the contaminants in NGS and molecular biology workflows. It can be used on samples after DNA isolation, fragmentation, PCR amplification, cloning, library preparation, etc. With proprietary Apostle MiniEnrichTM technology, the kit is featured for its more efficient removal of contaminants and recovery of DNA fragments of interest than current market-leading products.

Performance Evaluation

Apostle MiniEnrichTM Purification Beads is designed to efficiently removes small fragments like primer, primer-dimer, adaptor, and adaptor-dimer, as well as other contaminates like dNTPs, salts and enzymes, without compromising the yield of DNA fragments of interest. The more efficient removal of short fragments for PCR clean-up in library preparation work will improve sequencing efficiency and reduce cost (Exhibit 4, 5, and 6).

Apostle MiniEnrich data image

Exhibit 4. More efficient removal of fragments less than 150 bp for PCR purification.

dsDNA step ladder (20-1000 bp) in a TE buffer was treated with Apostle MiniEnrichTM Purification Beads (blue) or a magnetic beads-based market-leading product (red). Apostle MiniEnrichTM Purification Beads provides more efficient removal of short fragments (<150 bp), while preserves long fragments (>150 bp) with high yield. This data demonstrates that Apostle MiniEnrichTM Purification Beads can be used to remove excessive primers (20-30 bp), primer-dimers (40-60 bp), adaptors (50-60 bp), and adaptor-dimers (100-120bp), without compromising the yield of fragments of interest during molecular biology and NGS workflow.

Exhibit 5. More efficient adaptor-dimer removal than competitors for cfDNA library preparation.

cfDNA isolated from plasma was processed with NEBNext® UltraTM II DNA Library Prep Kit for library preparation. The purification for adaptor-ligation step and final PCR product was performed using Apostle MiniEnrichTM Purification Beads (blue) or a magnetic beads-based market-leading product (red). Comparing to the market-leading product, Apostle MiniEnrichTM Purification Beads provides more efficient removal of excessive adaptor-dimers (120bp), while preserves the ligated cfDNA with high yield.
Apostle MiniEnrich data image

Apostle MiniEnrich data image

Exhibit 6. Efficient removal of adaptor and adaptor-dimers for gDNA library preparation.

Fragmented gDNA was processed with NEBNext® UltraTM II DNA Library Prep Kit for library preparation. The purification for adaptor-ligation step and final PCR product was performed using Apostle MiniEnrichTM Purification Beads (red). Compared with before cleaning up (blue), Apostle MiniEnrichTM Purification Beads provides efficient removal of excessive adaptors (60 bp) and adaptor-dimers (120bp),while preserves the ligated gDNA with high yield.