Apostle MagTouch 1000 Nucleic Acids Extraction Automation System


MagTouch 1000

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Overview
  1. High-throughput automation platform for nucleic acid extraction
  2. Compatible with Apostle MiniMax® High Efficiency cfDNA Isolation Kit and Apostle MiniGenomics® Genomic DNA isolation Kit
  3. 24-channel large volume samples per run for cfDNA isolation and 96-channel small volume samples per run for gDNA isolation
  4. Turntable for up to 8 plates
  5. Flexible working volume per well: 50 ~ 5000 µL
  6. Compatible with processing 50 – 6000 µL sample volume
  7. Working time per run: ~ 30 – 90 mins
  8. Dimension: 610*455*640 mm
Environmental Requirements
  1. Surrounding temperature: 10℃-40℃
  2. Humidity below 80%
  3. Voltage requirements: AC 110V– 230V, 50 Hz/60Hz, 300W
  4. Operation desk: Bearing weight beyond 50 kg
Features
MagTouch 1000
  1. Touchscreen operation and one-button start
  2. Build-in UV light for sterilization
  3. High-efficiency and high-throughput extraction
  4. Programmable procedure
  5. Novel magnetic field settings for high recovery of magnetic beads
  6. Five different mixing speed
  7. Vibration extent of the magnetic head automatically matches the reagent volume during mixing
  8. Precise temperature control within 25-80°C
Modules
Number Modules Description of Functions
1 Plastic tip comb module Mixing; transfer magnetic beads
2 Magnetic head module Magnetization of beads
3 Turntable module Switch of plates
4 Thermal module (optional Temperature control
5 Shield module Prevent potential cross contamination
6 UV sterilization module Sterilization
7 Working lamp module Illumination
Performance and Data
  1. Extraction efficiency
  2. Comparison between manual and automated extraction
  3. Consistency within batch/group of automated extraction
  4. Consistency between group/batches of automated extraction
  5. Comparison with a competing technology

1. Extraction efficiency



Apostle Magtouch 1000

Figure 1A

Apostle Magtouch 1000

Figure 1B

Figure 1: Extraction efficiency - Standard EGFR fragment with size around 170 bp containing L858R mutation was spiked into nucleic acid-free FBS, which was then equally spilt into 6 portions. 5 portions were extracted automatically by Apostle MagTouch 1000 and 1 portion was extracted manually. All extracts were analyzed by q-PCR to evaluate the recovery rate of EGFR fragment. Both manual extraction and automated extraction achieved high efficiency extraction of EGFR fragment.

2. Comparison between manual and automated extraction

Standard DNA ladder was spiked into nucleic acid-free FBS. The sample was equally split into 7 portions and the volume of each portion was 4 mL. 6 portions were automatically extracted by Apostle MagTouch 1000 and 1 portion was manually extracted. The results are shown as below:
No. Conc. (ng/μL) Volume (μL) Amount (ng) CV Ratio of automated extraction to manual extraction
Auto-1 1.45 100 145 3.0% 94.2%
Auto-2 1.43 100 143 92.9%
Auto-3 1.45 100 145 94.2%
Auto-4 1.49 100 149 96.8%
Auto-5 1.43 100 143 92.9%
Auto-6 1.41 100 141 91.6%
Manual 1.54 100 154 -

3. Consistency within batch/group of automated extraction

Apostle Magtouch 1000

Figure 2A

Apostle Magtouch 1000

Figure 2B

Figure 2: Consistency within batch of automated extraction - Standard DNA ladder was spiked into nucleic acid-free FBS. The sample was equally split into 3 portions, which were then added into 3 different wells (C5, B6, C6) within the 24-well plate, respectively, for the automated extraction by Apostle MagTouch 1000. The extracts were analyzed by Agilent Bioanalyzer 2100 to evaluate the well-to-well consistency, showing an excellent well-to-well reproducibility,with a CV<6%.

4. Consistency between group/batches of automated extraction

Standard DNA ladder was spiked into nucleic acid-free FBS. The sample was equally split into 9 portions. The 9 portions were then equally divided for 3 batches to evaluate the consistency among batches of automated extraction by Apostle MagTouch 1000. The data shows an excellent batch-to-batch reproducibility,with a CV<4%.
Well# Batch# Conc. (ng/μL) Volume(μL) Amount (ng) CV
1 1 1.33 100 133 3.0%
2 1.24 100 124
3 1.31 100 131
2 1 1.34 100 134 3.3%
2 1.29 100 129
3 1.39 100 139
3 1 1.32 100 132 0.4%
2 1.31 100 131
3 1.31 100 131

5. Comparison with a competing technology

Apostle’s automated extraction solution (Apostle MagTouch 1000 Automated machine + Apostle MiniMax ®High Efficiency cfDNA Isolation Kit) was compared side by side with a competing technology (Competitor’s Automated Purification System + Competitor’s Cell-Free DNA Isolation Kit) for the extraction of cfDNA from human plasma by two independent institutions.
Apostle Magtouch 1000
Apostle Magtouch 1000

Figure 3: Comparison with a competing technology for the extraction of cfDNA. These data indicate that Apostle’s automated extraction solution shows a better performance than a competing technology.